Spore Testing

Spore Testing

1st Jul 2024

An OSHA inspector may want to know ...

"Is Your Sterilizer/Autoclave Killing Bacteria?"

If you’re sterilizing your instruments inside an autoclave without using a spore test (a biological indicator) you’re placing your practice at risk. Without a spore test you don't know for sure if your autoclave is killing bacteria.

How Sterilization Takes Place
Proper sterilization takes place at 250°F for 40 minutes and 273°F for 20 minutes, plus dry time. At 250°F, pressure should be between 16-18 psi and 30-32 psi at 273°F.

"I Don’t Need Spore Testing - I Use Indicator Tape"
If this is your reaction, you may be surprised to find out indicator tape will change colors when they reach a certain temperature - however, they do not tell you if the proper temperature was reached for the right amount of time or if the pressure was correct for sterilization to take place. Only spore testing will tell.

Who Requires Spore Testing?
Since spore testing is the only reliable method for verifying that the sterilization process is actually destroying microorganisms and spores, it is recommended weekly by: OSHA, AAMI, CDC, JCAHO, AORN, ADA, AHA and many insurance companies. (For clarification of OSHA guidelines feel free to call Dr. Sheila Dunn, OSHA & Laboratory Consultant, at 828-645-3661.)

How Does Spore Testing Work?

  • We mail you a packet of non-pathogenic spore strips
  • You pack it with your instruments and autoclave as usual
  • You return it to us
  • Upon completion of culturing, a certificate of test records is sent to you in writing for your records
  • In the event of any adverse results, your are advised immediately and confidentially by phone

    Your Added Protection...
    Sporicidal testing is the only proof that your autoclave and it's operator are doing the best possible job.

    Start Right Away!
    Protect yourself, your practice and your patients with a small investment in safety by using spore testing regularly in your autoclave.

    Type of Service:

    • Monthly Spore Testing ($175) - Includes 12 test strips (1 per month), reminder stickers, and culturing service at our laboratory.
    • Weekly Spore Testing ($420) - Includes 52 test strips (4 per month), reminder stickers, and culturing service at our laboratory.